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KMID : 0360220170580101155
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Journal of the Korean Ophthalmological Society
2017 Volume.58 No. 10 p.1155 ~ p.1159
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The Effect of Anti-inflammatory Agents on the Permeability of Trabecular Meshwork Cell Monolayers
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Kang Hye-Ri
Lee Jung-Hoon
Kim Jae-Woo
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Abstract
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Purpose: To compare the effects of anti-inflammatory agents, specifically bromfenac, loteprednol, and prednisolone, on the permeability of cultured human trabecular meshwork cell (HTMC) monolayers.
Methods: HTMCs were cultured until confluency in the inner chamber of Transwell, then exposed to 1/1,000 or 1/500 diluted commercial 0.1% bromfenac, 0.5% loteprednol, and 1% prednisolone for 24 hours. The permeabilities of carboxyfluorescein through the HTMC monolayer were measured with a spectrofluorometer after 2 hours in the outer chamber. Cellular viabilities were assessed with an 3-[4,5?dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay.
Results: Bromfenac and loteprednol diluted at 1/1,000 or 1/500 did not significantly affect the cellular survival (p > 0.05). Bromfenac did not affect the permeability via the HTMC monolayer (p > 0.05) and loteprednol decreased the permeability (p < 0.05). In addition, 1/2,000 prednisolone also decreased the permeability (p < 0.05).
Conclusions: Among the anti-inflammatory agents, the non-steroidal anti-inflammatory agent bromfenac did not affect the permeability, while loteprednol and prednisolone decreased the permeability through the HTMC monolayer. Thus, loteprednol and prednisolone may decrease the trabecular outflow.
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KEYWORD
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Bromfenac, Loteprednol, Permeability, Prednisolone, Trabecular meshwork cells
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